Genome-wide identification and expression pattern analysis of the GRF transcription factor family in Astragalus mongholicus.
Gene Expression Regulation, Plant
/ genetics
Plant Proteins
/ genetics
Phylogeny
Transcription Factors
/ genetics
Astragalus propinquus
/ genetics
Multigene Family
Genome, Plant
Gene Expression Profiling
/ methods
Promoter Regions, Genetic
/ genetics
Astragalus Plant
/ genetics
Plant Roots
/ genetics
Stress, Physiological
/ genetics
Transcriptome
/ genetics
Plant Growth Regulators
/ metabolism
Astragalus mongholics
Cis-acting elements
Evolutionary analysis
Expression pattern
Growth-regulating factor
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
06 May 2024
06 May 2024
Historique:
received:
08
02
2024
accepted:
22
04
2024
medline:
6
5
2024
pubmed:
6
5
2024
entrez:
5
5
2024
Statut:
epublish
Résumé
Astragalus membranaceus is a plant of the Astragalus genus, which is used as a traditional Chinese herbal medicine with extremely high medicinal and edible value. Astragalus mongholicus, as one of the representative medicinal materials with the same origin of medicine and food, has a rising market demand for its raw materials, but the quality is different in different production areas. Growth-regulating factors (GRF) are transcription factors unique to plants that play important roles in plant growth and development. Up to now, there is no report about GRF in A. mongholicus. This study conducted a genome-wide analysis of the AmGRF gene family, identifying a total of nine AmGRF genes that were classified into subfamily V based on phylogenetic relationships. In the promoter region of the AmGRF gene, we successfully predicted cis-elements that respond to abiotic stress, growth, development, and hormone production in plants. Based on transcriptomic data and real-time quantitative polymerase chain reaction (qPCR) validation, the results showed that AmGRFs were expressed in the roots, stems, and leaves, with overall higher expression in leaves, higher expression of AmGRF1 and AmGRF8 in roots, and high expression levels of AmGRF1 and AmGRF9 in stems. The results of this study provide a theoretical basis for the further exploration of the functions of AmGRFs in plant growth and development.
Sections du résumé
BACKGROUND
BACKGROUND
Astragalus membranaceus is a plant of the Astragalus genus, which is used as a traditional Chinese herbal medicine with extremely high medicinal and edible value. Astragalus mongholicus, as one of the representative medicinal materials with the same origin of medicine and food, has a rising market demand for its raw materials, but the quality is different in different production areas. Growth-regulating factors (GRF) are transcription factors unique to plants that play important roles in plant growth and development. Up to now, there is no report about GRF in A. mongholicus.
METHODS AND RESULTS
RESULTS
This study conducted a genome-wide analysis of the AmGRF gene family, identifying a total of nine AmGRF genes that were classified into subfamily V based on phylogenetic relationships. In the promoter region of the AmGRF gene, we successfully predicted cis-elements that respond to abiotic stress, growth, development, and hormone production in plants. Based on transcriptomic data and real-time quantitative polymerase chain reaction (qPCR) validation, the results showed that AmGRFs were expressed in the roots, stems, and leaves, with overall higher expression in leaves, higher expression of AmGRF1 and AmGRF8 in roots, and high expression levels of AmGRF1 and AmGRF9 in stems.
CONCLUSIONS
CONCLUSIONS
The results of this study provide a theoretical basis for the further exploration of the functions of AmGRFs in plant growth and development.
Identifiants
pubmed: 38705956
doi: 10.1007/s11033-024-09581-8
pii: 10.1007/s11033-024-09581-8
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Plant Growth Regulators
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
618Subventions
Organisme : wei ma
ID : (ZYRCB2021008); No.2021YFD1600901;821713927;[2019] No. 5)
Organisme : wei ma
ID : (ZYRCB2021008); No.2021YFD1600901;821713927;[2019] No. 5)
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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