Characterization and expression analysis of the MADS-box gene AGL8 in cotton: insights into gene function differentiation in plant growth and stress resistance.
Gossypium
/ genetics
Gene Expression Regulation, Plant
MADS Domain Proteins
/ genetics
Phylogeny
Plant Proteins
/ genetics
Stress, Physiological
/ genetics
Plant Growth Regulators
/ pharmacology
Gene Expression Profiling
/ methods
Promoter Regions, Genetic
/ genetics
Cyclopentanes
/ pharmacology
Oxylipins
/ pharmacology
Plant Development
/ genetics
Salicylic Acid
/ pharmacology
Transcriptome
/ genetics
Acetates
AGL8
Abiotic stress
Cotton
Genome analysis
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
04 Oct 2024
04 Oct 2024
Historique:
received:
12
05
2024
accepted:
03
09
2024
medline:
4
10
2024
pubmed:
4
10
2024
entrez:
4
10
2024
Statut:
epublish
Résumé
AGAMOUS-LIKE 8 (AGL8) belongs to the MADS-box family, which plays important roles in transcriptional regulation, sequence-specific DNA binding and other biological processes and molecular functions. The genome of cotton, a representative polyploid plant, contains multiple AGL8 genes. However, their functional differentiation is still unclear. In this study, a comprehensive genomic analysis of AGL8 genes was conducted. Cotton AGL8s were subdivided into four subgroups (Groups 1, 2, 3, and 4) based on phylogenetic analysis, and different subgroups of AGL8s presented different characteristics, including different structures and conserved motifs. With respect to the promoter regions of the GhAGL8 genes, we successfully predicted cis-elements that respond to phytohormone signal transduction and the stress response of plants. Transcriptome data and real-time quantitative PCR validation indicated that three genes, namely, GH_D07G0744, GH_A03G0856 and GH_A07G0749, were highly induced by methyl jasmonate (MeJA), salicylic acid (SA), and abscisic acid (ABA), which indicated that they function in plant resistance to abiotic and biotic stresses. The information from the gene structure, number and types of conserved domains, tissue-specific expression levels, and expression patterns under different treatments highlights the differences in sequence and function of the cotton AGL8 genes. Different AGL8s play roles in vegetative growth, reproductive development, and plant stress resistance. These results lay a foundation for further study of GhAGL8s in cotton.
Sections du résumé
BACKGROUND
BACKGROUND
AGAMOUS-LIKE 8 (AGL8) belongs to the MADS-box family, which plays important roles in transcriptional regulation, sequence-specific DNA binding and other biological processes and molecular functions. The genome of cotton, a representative polyploid plant, contains multiple AGL8 genes. However, their functional differentiation is still unclear.
METHODS AND RESULTS
RESULTS
In this study, a comprehensive genomic analysis of AGL8 genes was conducted. Cotton AGL8s were subdivided into four subgroups (Groups 1, 2, 3, and 4) based on phylogenetic analysis, and different subgroups of AGL8s presented different characteristics, including different structures and conserved motifs. With respect to the promoter regions of the GhAGL8 genes, we successfully predicted cis-elements that respond to phytohormone signal transduction and the stress response of plants. Transcriptome data and real-time quantitative PCR validation indicated that three genes, namely, GH_D07G0744, GH_A03G0856 and GH_A07G0749, were highly induced by methyl jasmonate (MeJA), salicylic acid (SA), and abscisic acid (ABA), which indicated that they function in plant resistance to abiotic and biotic stresses.
CONCLUSIONS
CONCLUSIONS
The information from the gene structure, number and types of conserved domains, tissue-specific expression levels, and expression patterns under different treatments highlights the differences in sequence and function of the cotton AGL8 genes. Different AGL8s play roles in vegetative growth, reproductive development, and plant stress resistance. These results lay a foundation for further study of GhAGL8s in cotton.
Identifiants
pubmed: 39365489
doi: 10.1007/s11033-024-09902-x
pii: 10.1007/s11033-024-09902-x
doi:
Substances chimiques
MADS Domain Proteins
0
Plant Proteins
0
Plant Growth Regulators
0
Cyclopentanes
0
methyl jasmonate
900N171A0F
Oxylipins
0
Salicylic Acid
O414PZ4LPZ
Acetates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1037Subventions
Organisme : Initial Foundation of ZAFU
ID : 2023LFR028
Organisme : Natural Science Foundation of Zhejiang
ID : Y21C130006
Organisme : Natural Science Foundation of China
ID : 32171923
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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