Transcriptomic and proteomic effects of (-)-epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3"Me) treatment on ethanol-stressed Saccharomyces cerevisiae cells.
Cell Wall
/ drug effects
Chromatography, Liquid
Ethanol
/ adverse effects
Fermentation
Gallic Acid
/ analogs & derivatives
Gene Expression Profiling
Gene Expression Regulation
Glycolysis
Heat-Shock Proteins
/ genetics
Microscopy, Electron, Scanning
Oxidation-Reduction
Polyphenols
/ pharmacology
Proteomics
Reactive Oxygen Species
/ metabolism
Reproducibility of Results
Saccharomyces cerevisiae
/ drug effects
Saccharomyces cerevisiae Proteins
/ genetics
Sequence Analysis, RNA
Stress, Physiological
/ genetics
Tandem Mass Spectrometry
Tea
/ chemistry
Transcriptome
/ genetics
Wine
/ microbiology
EGCG3”Me
Ethanol stress
RNA-seq transcriptomic
Saccharomyces cerevisiae
iTRAQ proteomic
Journal
Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
13
10
2018
revised:
22
01
2019
accepted:
23
01
2019
entrez:
20
3
2019
pubmed:
20
3
2019
medline:
14
7
2020
Statut:
ppublish
Résumé
Saccharomyces cerevisiae (S. cerevisiae) is the main fermentation strain in brewing industry. However, the accumulation of ethanol during the fermentation inhibits the growth of S. cerevisiae. Polyphenols are important bioactive ingredients in oolong tea, and epigallocatechin-3-O-(3"-O-methyl)-gallate (EGCG3"Me) has exhibited ameliorate effect on alcohol intoxication. Therefore, in the current work, we used RNA-seq transcriptomics and iTRAQ proteomic analysis to study the effect of EGCG3"Me on ethanol-stressed S. cerevisiae. After EGCG3"Me intervention (0.8%, w/v), 178 up-regulated and 172 down-regulated genes were identified, meanwhile, 190 differentially expressed proteins (DEPs) were identified. In addition, KEGG pathways for metabolic pathways, biosynthesis of secondary metabolites and microbial metabolism were among the most DEPs after EGCG3"Me intervention. The integrated transcriptomic and proteomic analysis indicated EGCG3"Me may alleviate ethanol-induced damage on the cell wall and cell membrane of S. cerevisiae, and facilitate the redox balance and glycolysis. This study provides new insights into the mechanisms underlying the molecular response to ethanol in S. cerevisiae by the treatment of EGCG3"Me.
Identifiants
pubmed: 30884702
pii: S0963-9969(19)30070-5
doi: 10.1016/j.foodres.2019.01.061
pii:
doi:
Substances chimiques
Heat-Shock Proteins
0
Polyphenols
0
Reactive Oxygen Species
0
Saccharomyces cerevisiae Proteins
0
Tea
0
Ethanol
3K9958V90M
Gallic Acid
632XD903SP
epigallocatechin-3-O-(3''-O-methyl)-gallate
O97U9TPY8V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
67-75Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.