Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using next-generation sequencing.
Cactus
Endangered species
Microsatellites
Population genetics
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
19
07
2019
accepted:
28
11
2019
pubmed:
11
12
2019
medline:
8
10
2020
entrez:
11
12
2019
Statut:
ppublish
Résumé
Ten microsatellite loci were developed and validated for the endangered cactus species Coleocephalocereus purpureus. The markers were obtained from sequences generated by whole genome shotgun sequencing approaches. A testing group of 36 specimens of the main population were genotyped and all described markers presented suitable outcomes to population genetic studies, showing polymorphic status for C. purpureus testing group with clean and reproducible amplification. No evidence for scoring errors, null alleles or linkage disequilibrium was detected. Number of alleles per locus ranged from 3 to 6 and expected heterozygosity ranged from 0.78 to 0.99. These new microsatellite loci are suitable to be used in future diversity and structure population studies of C. purpureus.
Identifiants
pubmed: 31820311
doi: 10.1007/s11033-019-05213-8
pii: 10.1007/s11033-019-05213-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1485-1489Subventions
Organisme : Instituto Federal de Minas Gerais
ID : Edital de Pesquisa Aplicada (Edital 104-2016)
Organisme : FAPEMIG
ID : Processo: MPR-01008-16
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