Allopatric divergence and hybridization within Cupressus chengiana (Cupressaceae), a threatened conifer in the northern Hengduan Mountains of western China.
Cell Nucleus
/ genetics
China
Conservation of Natural Resources
Cupressus
/ genetics
DNA, Chloroplast
/ genetics
Ecosystem
Endangered Species
Evolution, Molecular
Gene Flow
Genetics, Population
High-Throughput Nucleotide Sequencing
Hybridization, Genetic
Phylogeny
Polymorphism, Single Nucleotide
RNA-Seq
Transcriptome
evolutionarily significant units
hybridization
mountainous regions
population genomics
threatened species
Journal
Molecular ecology
ISSN: 1365-294X
Titre abrégé: Mol Ecol
Pays: England
ID NLM: 9214478
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
13
09
2019
revised:
21
02
2020
accepted:
26
02
2020
pubmed:
10
3
2020
medline:
2
3
2021
entrez:
10
3
2020
Statut:
ppublish
Résumé
Having a comprehensive understanding of population structure, genetic differentiation and demographic history is important for the conservation and management of threatened species. High-throughput sequencing (HTS) provides exciting opportunities to address a wide range of factors for conservation genetics. Here, we generated HTS data and identified 266,884 high-quality single nucleotide polymorphisms from 82 individuals of Cupressus chengiana, to assess population genomics across the species' full range, comprising the Daduhe River (DDH), Minjiang River (MJR) and Bailongjiang River (BLJ) catchments in western China. admixture, principal components analysis and phylogenetic analyses indicated that each region contains a distinct lineage, with high levels of differentiation between them (DDH, MJR and BLJ lineages). MJR was newly distinguished compared to previous surveys, and evidence including coalescent simulations supported a hybrid origin of MJR during the Quaternary. Each of these three lineages should be recognized as an evolutionarily significant unit (ESU), due to isolation, differing genetic adaptations and different demographic history. Currently, each ESU faces distinct threats, and will require different conservation strategies. Our work shows that population genomic approaches using HTS can reconstruct the complex evolutionary history of threatened species in mountainous regions, and hence inform conservation efforts, and contribute to the understanding of high biodiversity in mountains.
Substances chimiques
DNA, Chloroplast
0
Banques de données
GENBANK
['NC_036939.1']
Dryad
['10.5061/dryad.70rxwdbtf']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1250-1266Informations de copyright
© 2020 John Wiley & Sons Ltd.
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