ExpansionHunter Denovo: a computational method for locating known and novel repeat expansions in short-read sequencing data.


Journal

Genome biology
ISSN: 1474-760X
Titre abrégé: Genome Biol
Pays: England
ID NLM: 100960660

Informations de publication

Date de publication:
28 04 2020
Historique:
received: 20 12 2019
accepted: 14 04 2020
entrez: 30 4 2020
pubmed: 30 4 2020
medline: 24 2 2021
Statut: epublish

Résumé

Repeat expansions are responsible for over 40 monogenic disorders, and undoubtedly more pathogenic repeat expansions remain to be discovered. Existing methods for detecting repeat expansions in short-read sequencing data require predefined repeat catalogs. Recent discoveries emphasize the need for methods that do not require pre-specified candidate repeats. To address this need, we introduce ExpansionHunter Denovo, an efficient catalog-free method for genome-wide repeat expansion detection. Analysis of real and simulated data shows that our method can identify large expansions of 41 out of 44 pathogenic repeats, including nine recently reported non-reference repeat expansions not discoverable via existing methods.

Identifiants

pubmed: 32345345
doi: 10.1186/s13059-020-02017-z
pii: 10.1186/s13059-020-02017-z
pmc: PMC7187524
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102

Subventions

Organisme : CIHR
Pays : Canada

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Auteurs

Egor Dolzhenko (E)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA.

Mark F Bennett (MF)

Population Health and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia.
Department of Medical Biology, The University of Melbourne, 1G Royal Parade, Parkville, VIC, 3052, Australia.
Epilepsy Research Centre, Department of Medicine, The University of Melbourne, Austin Health, 245 Burgundy Street, Heidelberg, VIC, 3084, Australia.

Phillip A Richmond (PA)

Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, University of British Columbia, Vancouver, BC, V5Z 4H4, Canada.

Brett Trost (B)

Genetics and Genome Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
The Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.

Sai Chen (S)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA.

Joke J F A van Vugt (JJFA)

Department of Neurology, UMC Utrecht Brain Center, Utrecht University, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.

Charlotte Nguyen (C)

Genetics and Genome Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
The Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON, M5S 2E5, Canada.

Giuseppe Narzisi (G)

New York Genome Center, 101 Avenue of the Americas, New York, 10013, USA.

Vladimir G Gainullin (VG)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA.

Andrew M Gross (AM)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA.

Bryan R Lajoie (BR)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA.

Ryan J Taft (RJ)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA.

Wyeth W Wasserman (WW)

Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, University of British Columbia, Vancouver, BC, V5Z 4H4, Canada.

Stephen W Scherer (SW)

Genetics and Genome Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
The Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON, M5S 2E5, Canada.
The McLaughlin Centre, University of Toronto, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.

Jan H Veldink (JH)

Department of Neurology, UMC Utrecht Brain Center, Utrecht University, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.

David R Bentley (DR)

Illumina Cambridge Ltd, Illumina Centre, 19 Granta Park, Great Abington, Cambridge, CB21 6DF, UK.

Ryan K C Yuen (RKC)

Genetics and Genome Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
The Centre for Applied Genomics, The Hospital for Sick Children, 686 Bay Street, Toronto, ON, M5G 0A4, Canada.
Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON, M5S 2E5, Canada.

Melanie Bahlo (M)

Population Health and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC, 3052, Australia.
Department of Medical Biology, The University of Melbourne, 1G Royal Parade, Parkville, VIC, 3052, Australia.

Michael A Eberle (MA)

Illumina Inc., 5200 Illumina Way, San Diego, CA, 92122, USA. meberle@illumina.com.

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Classifications MeSH