Evolution of Fusarium tricinctum and Fusarium avenaceum mitochondrial genomes is driven by mobility of introns and of a new type of palindromic microsatellite repeats.


Journal

BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258

Informations de publication

Date de publication:
12 May 2020
Historique:
received: 17 02 2020
accepted: 04 05 2020
entrez: 14 5 2020
pubmed: 14 5 2020
medline: 13 1 2021
Statut: epublish

Résumé

Increased contamination of European and Asian wheat and barley crops with "emerging" mycotoxins such as enniatins or beauvericin, produced by Fusarium avenaceum and Fusarium tricinctum, suggest that these phylogenetically close species could be involved in future food-safety crises. The mitochondrial genomes of F. tricinctum strain INRA104 and F. avenaceum strain FaLH27 have been annotated. A comparative analysis was carried out then extended to a set of 25 wild strains. Results show that they constitute two distinct species, easily distinguished by their mitochondrial sequences. The mitochondrial genetic variability is mainly located within the intergenic regions. Marks of variations show they have evolved (i) by Single Nucleotide Polymorphisms (SNPs), (ii) by length variations mediated by insertion/deletion sequences (Indels), and (iii) by length mutations generated by DNA sliding events occurring in mononucleotide (A) Mitochondrial genome evolution between F. tricinctum and F. avenaceum is mostly driven by two types of mobile genetic elements, implicated in genome polymorphism. The first one is represented by group I introns. Indeed, both genomes harbour optional (inter- or intra-specifically) group I introns, all carrying putatively functional hegs, arguing for a high mobility of these introns during short evolution periods. The gain events were shown to involve, for most of them, lateral transfers between phylogenetically distant species. This study has also revealed a new type of mobile genetic element constituted by a palindromic arrangement of (A) n and (T) n microsatellite sequences whose presence was related to occurrence of SNPs and Indels in the neighbouring regions.

Sections du résumé

BACKGROUND BACKGROUND
Increased contamination of European and Asian wheat and barley crops with "emerging" mycotoxins such as enniatins or beauvericin, produced by Fusarium avenaceum and Fusarium tricinctum, suggest that these phylogenetically close species could be involved in future food-safety crises.
RESULTS RESULTS
The mitochondrial genomes of F. tricinctum strain INRA104 and F. avenaceum strain FaLH27 have been annotated. A comparative analysis was carried out then extended to a set of 25 wild strains. Results show that they constitute two distinct species, easily distinguished by their mitochondrial sequences. The mitochondrial genetic variability is mainly located within the intergenic regions. Marks of variations show they have evolved (i) by Single Nucleotide Polymorphisms (SNPs), (ii) by length variations mediated by insertion/deletion sequences (Indels), and (iii) by length mutations generated by DNA sliding events occurring in mononucleotide (A)
CONCLUSIONS CONCLUSIONS
Mitochondrial genome evolution between F. tricinctum and F. avenaceum is mostly driven by two types of mobile genetic elements, implicated in genome polymorphism. The first one is represented by group I introns. Indeed, both genomes harbour optional (inter- or intra-specifically) group I introns, all carrying putatively functional hegs, arguing for a high mobility of these introns during short evolution periods. The gain events were shown to involve, for most of them, lateral transfers between phylogenetically distant species. This study has also revealed a new type of mobile genetic element constituted by a palindromic arrangement of (A) n and (T) n microsatellite sequences whose presence was related to occurrence of SNPs and Indels in the neighbouring regions.

Identifiants

pubmed: 32397981
doi: 10.1186/s12864-020-6770-2
pii: 10.1186/s12864-020-6770-2
pmc: PMC7218506
doi:

Substances chimiques

Fungal Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

358

Subventions

Organisme : National key research and development program of China
ID : 2016YFD0501207

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Auteurs

Nadia Ponts (N)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Charlotte Gautier (C)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Jérôme Gouzy (J)

LIPM, Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.

Laetitia Pinson-Gadais (L)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Marie Foulongne-Oriol (M)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Christine Ducos (C)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Florence Richard-Forget (F)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Jean-Michel Savoie (JM)

INRAE, MycSA, F-33882, Villenave d'Ornon, France.

Chen Zhao (C)

Academy of National Food and Strategic Reserves Administration, Beijing, China.

Gérard Barroso (G)

INRAE, MycSA, F-33882, Villenave d'Ornon, France. gerard.barroso@u-bordeaux.fr.
University of Bordeaux, INRAE, MycSA, F-33882, Villenave d'Ornon, France. gerard.barroso@u-bordeaux.fr.

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Classifications MeSH