Genome-wide identification and expression analysis of aquaporin family in Canavalia rosea and their roles in the adaptation to saline-alkaline soils and drought stress.
Adaptation, Physiological
Amino Acid Motifs
Aquaporins
/ genetics
Biological Evolution
Canavalia
/ genetics
Chromosome Mapping
Chromosomes, Plant
Droughts
Ecosystem
Genome, Plant
Multigene Family
Plant Proteins
/ genetics
Promoter Regions, Genetic
RNA-Seq
Soil
/ chemistry
Stress, Physiological
Transcriptome
Aquaporin
Canavalia rosea (Sw.) DC.
Drought
Saline-alkaline soil
Water deficit
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
13 Jul 2021
13 Jul 2021
Historique:
received:
12
01
2021
accepted:
03
05
2021
entrez:
14
7
2021
pubmed:
15
7
2021
medline:
29
7
2021
Statut:
epublish
Résumé
Canavalia rosea (Sw.) DC. (bay bean) is an extremophile halophyte that is widely distributed in coastal areas of the tropics and subtropics. Seawater and drought tolerance in this species may be facilitated by aquaporins (AQPs), channel proteins that transport water and small molecules across cell membranes and thereby maintain cellular water homeostasis in the face of abiotic stress. In C. rosea, AQP diversity, protein features, and their biological functions are still largely unknown. We describe the action of AQPs in C. rosea using evolutionary analyses coupled with promoter and expression analyses. A total of 37 AQPs were identified in the C. rosea genome and classified into five subgroups: 11 plasma membrane intrinsic proteins, 10 tonoplast intrinsic proteins, 11 Nod26-like intrinsic proteins, 4 small and basic intrinsic proteins, and 1 X-intrinsic protein. Analysis of RNA-Seq data and targeted qPCR revealed organ-specific expression of aquaporin genes and the involvement of some AQP members in adaptation of C. rosea to extreme coral reef environments. We also analyzed C. rosea sequences for phylogeny reconstruction, protein modeling, cellular localizations, and promoter analysis. Furthermore, one of PIP1 gene, CrPIP1;5, was identified as functional using a yeast expression system and transgenic overexpression in Arabidopsis. Our results indicate that AQPs play an important role in C. rosea responses to saline-alkaline soils and drought stress. These findings not only increase our understanding of the role AQPs play in mediating C. rosea adaptation to extreme environments, but also improve our knowledge of plant aquaporin evolution more generally.
Sections du résumé
BACKGROUND
BACKGROUND
Canavalia rosea (Sw.) DC. (bay bean) is an extremophile halophyte that is widely distributed in coastal areas of the tropics and subtropics. Seawater and drought tolerance in this species may be facilitated by aquaporins (AQPs), channel proteins that transport water and small molecules across cell membranes and thereby maintain cellular water homeostasis in the face of abiotic stress. In C. rosea, AQP diversity, protein features, and their biological functions are still largely unknown.
RESULTS
RESULTS
We describe the action of AQPs in C. rosea using evolutionary analyses coupled with promoter and expression analyses. A total of 37 AQPs were identified in the C. rosea genome and classified into five subgroups: 11 plasma membrane intrinsic proteins, 10 tonoplast intrinsic proteins, 11 Nod26-like intrinsic proteins, 4 small and basic intrinsic proteins, and 1 X-intrinsic protein. Analysis of RNA-Seq data and targeted qPCR revealed organ-specific expression of aquaporin genes and the involvement of some AQP members in adaptation of C. rosea to extreme coral reef environments. We also analyzed C. rosea sequences for phylogeny reconstruction, protein modeling, cellular localizations, and promoter analysis. Furthermore, one of PIP1 gene, CrPIP1;5, was identified as functional using a yeast expression system and transgenic overexpression in Arabidopsis.
CONCLUSIONS
CONCLUSIONS
Our results indicate that AQPs play an important role in C. rosea responses to saline-alkaline soils and drought stress. These findings not only increase our understanding of the role AQPs play in mediating C. rosea adaptation to extreme environments, but also improve our knowledge of plant aquaporin evolution more generally.
Identifiants
pubmed: 34256694
doi: 10.1186/s12870-021-03034-1
pii: 10.1186/s12870-021-03034-1
pmc: PMC8278772
doi:
Substances chimiques
Aquaporins
0
Plant Proteins
0
Soil
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
333Informations de copyright
© 2021. The Author(s).
Références
Cells. 2018 Dec 11;7(12):
pubmed: 30545006
Curr Opin Plant Biol. 2014 Dec;22:101-107
pubmed: 25299641
Science. 2001 Dec 14;294(5550):2353-7
pubmed: 11743202
Proteins. 2010 Feb 15;78(3):661-70
pubmed: 19842162
Plant J. 2006 Feb;45(4):523-39
pubmed: 16441347
Plant Cell. 2003 Feb;15(2):439-47
pubmed: 12566583
Crit Rev Biotechnol. 2016;36(3):389-98
pubmed: 25430890
Nat Methods. 2015 Jan;12(1):59-60
pubmed: 25402007
J Plant Res. 2015 Jan;128(1):103-13
pubmed: 25358447
Science. 1992 Apr 17;256(5055):385-7
pubmed: 1373524
Plant Cell Rep. 2015 Aug;34(8):1401-15
pubmed: 25947559
PLoS One. 2015 Nov 12;10(11):e0142446
pubmed: 26562158
J Biotechnol. 2020 Dec 20;324:103-111
pubmed: 33007348
Genomics. 2020 Jan;112(1):263-275
pubmed: 30826442
Int J Mol Sci. 2019 Jan 10;20(2):
pubmed: 30634702
Plant Physiol Biochem. 2020 Apr;149:178-189
pubmed: 32078896
Int J Mol Sci. 2019 Aug 16;20(16):
pubmed: 31426275
Plant Sci. 2017 Nov;264:179-187
pubmed: 28969798
Recent Pat Food Nutr Agric. 2019;10(1):57-61
pubmed: 29984666
Biol Cell. 2010 Jan;103(1):35-54
pubmed: 21143194
Nature. 2000 Oct 5;407(6804):599-605
pubmed: 11034202
Biol Res. 2018 Jan 16;51(1):4
pubmed: 29338771
Plant Physiol. 2005 Sep;139(1):287-95
pubmed: 16113222
Plant Sci. 2019 Oct;287:110199
pubmed: 31481201
PLoS One. 2015 Sep 04;10(9):e0137447
pubmed: 26340746
Mol Plant. 2020 Aug 3;13(8):1194-1202
pubmed: 32585190
BMC Genomics. 2019 May 15;20(1):380
pubmed: 31092186
Plant Physiol. 2001 Mar;125(3):1206-15
pubmed: 11244102
Front Plant Sci. 2019 May 28;10:632
pubmed: 31191567
Biophys Rev. 2017 Oct;9(5):545-562
pubmed: 28871493
Plant Mol Biol. 2007 Aug;64(6):621-32
pubmed: 17522953
J Physiol. 2015 Dec 1;593(23):5025-8
pubmed: 26568076
Mol Biol Evol. 2002 Apr;19(4):456-61
pubmed: 11919287
Int J Mol Sci. 2018 Jul 30;19(8):
pubmed: 30061546
Nat Rev Drug Discov. 2014 Apr;13(4):259-77
pubmed: 24625825
Plant Physiol Biochem. 2020 Oct;155:743-755
pubmed: 32866789
Annu Rev Plant Biol. 2008;59:651-81
pubmed: 18444910
Front Plant Sci. 2012 Feb 20;3:33
pubmed: 22639644
Int J Mol Sci. 2019 Jan 03;20(1):
pubmed: 30609831
Mol Biol Evol. 1986 Sep;3(5):418-26
pubmed: 3444411
FEBS Lett. 2015 Nov 30;589(23):3508-15
pubmed: 26526614
J Exp Bot. 2012 Mar;63(5):2217-30
pubmed: 22223812
Int J Mol Sci. 2021 Jan 08;22(2):
pubmed: 33429984
Plant Cell Environ. 2008 May;31(5):658-66
pubmed: 18266903
PeerJ. 2019 Sep 12;7:e7664
pubmed: 31565576
Gene. 2018 Jul 30;665:41-48
pubmed: 29709638
Plant Physiol. 2001 Aug;126(4):1358-69
pubmed: 11500536
Front Plant Sci. 2016 Nov 29;7:1802
pubmed: 27965700
J Plant Physiol. 2011 Jul 15;168(11):1241-8
pubmed: 21397356
Planta. 2013 Oct;238(4):669-81
pubmed: 23801298
Plant Sci. 2014 Mar;217-218:71-7
pubmed: 24467898
Genes (Basel). 2019 May 22;10(5):
pubmed: 31121945
New Phytol. 2019 Feb;221(3):1197-1214
pubmed: 30222198
Front Plant Sci. 2018 Mar 26;9:382
pubmed: 29632543
Int J Mol Sci. 2015 Aug 20;16(8):19728-51
pubmed: 26307965
Int J Mol Sci. 2017 Oct 27;18(11):
pubmed: 29077056
Plant J. 2004 Jan;37(2):147-55
pubmed: 14690500
Nature. 2006 Feb 9;439(7077):688-94
pubmed: 16340961
Biol Cell. 2005 Oct;97(10):749-64
pubmed: 16171457
Physiol Rev. 2015 Oct;95(4):1321-58
pubmed: 26336033
Plant Cell Rep. 2016 Feb;35(2):385-95
pubmed: 26581952