The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei.
Amino Acid Sequence
Biological Evolution
Cell Cycle
/ genetics
Cell Nucleolus
/ genetics
Cloning, Molecular
DEAD-box RNA Helicases
/ chemistry
Gene Expression Regulation
Gene Knockdown Techniques
Organelle Biogenesis
Protein Transport
Ribosomes
/ metabolism
Sequence Analysis, DNA
Trypanosoma brucei brucei
/ genetics
Trypanosomiasis, African
/ parasitology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 09 2021
15 09 2021
Historique:
received:
11
06
2021
accepted:
30
07
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process.
Identifiants
pubmed: 34526538
doi: 10.1038/s41598-021-97020-0
pii: 10.1038/s41598-021-97020-0
pmc: PMC8443567
doi:
Substances chimiques
DEAD-box RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18325Informations de copyright
© 2021. The Author(s).
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