The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast.
Amino Acid Sequence
Cell Division
/ genetics
DNA-Binding Proteins
/ genetics
G1 Phase
/ genetics
Gene Expression Regulation, Fungal
Immunoblotting
Nitrogen
/ metabolism
Protein Binding
RNA-Seq
/ methods
Repressor Proteins
/ genetics
S Phase
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Sequence Homology, Amino Acid
Transcription Factors
/ genetics
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
21
12
2020
accepted:
19
01
2022
entrez:
3
3
2022
pubmed:
4
3
2022
medline:
22
3
2022
Statut:
epublish
Résumé
Commitment to cell division at the end of G1 phase, termed Start in the budding yeast Saccharomyces cerevisiae, is strongly influenced by nutrient availability. To identify new dominant activators of Start that might operate under different nutrient conditions, we screened a genome-wide ORF overexpression library for genes that bypass a Start arrest caused by absence of the G1 cyclin Cln3 and the transcriptional activator Bck2. We recovered a hypothetical gene YLR053c, renamed NRS1 for Nitrogen-Responsive Start regulator 1, which encodes a poorly characterized 108 amino acid microprotein. Endogenous Nrs1 was nuclear-localized, restricted to poor nitrogen conditions, induced upon TORC1 inhibition, and cell cycle-regulated with a peak at Start. NRS1 interacted genetically with SWI4 and SWI6, which encode subunits of the main G1/S transcription factor complex SBF. Correspondingly, Nrs1 physically interacted with Swi4 and Swi6 and was localized to G1/S promoter DNA. Nrs1 exhibited inherent transactivation activity, and fusion of Nrs1 to the SBF inhibitor Whi5 was sufficient to suppress other Start defects. Nrs1 appears to be a recently evolved microprotein that rewires the G1/S transcriptional machinery under poor nitrogen conditions.
Identifiants
pubmed: 35239649
doi: 10.1371/journal.pbio.3001548
pii: PBIOLOGY-D-20-03681
pmc: PMC8893695
doi:
Substances chimiques
DNA-Binding Proteins
0
Repressor Proteins
0
SWI4 protein, S cerevisiae
0
SWI6 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Transcription Factors
0
Whi5 protein, S cerevisiae
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3001548Subventions
Organisme : NIH HHS
ID : R01 OD010929
Pays : United States
Organisme : CIHR
ID : FDN-167277
Pays : Canada
Commentaires et corrections
Type : ErratumIn
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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